To make the links between the institutes that make up the NICR more effective and in order to participate in information and know-how sharing, it became necessary to create a catalogue of technologies and instruments. The purpose of this undertaking is to facilitate their future sharing: thanks to this initiative, research activities will not take place in isolation. Instead, they will be organically distributed throughout the consortium. The sharing of technologies and instruments will moreover contribute to a more effective use of resources needed for their acquisition. Take a look at whether some of the capacities on offer by various NICR institutes might be useful for your research!
CHARLES UNIVERSITY
First Faculty of Medicine
- Incucyte – automated live cell analysis in real time
Contact: lukas.lacina@lf1.cuni.cz
- timsTOF HT with NanoElute 2 and EvoSep chromatography – mass spectrometry with nanoLC separation
Contact: helena.skalnikova@lf1.cuni.cz
- xMAP Intelliflex DRSE – multiplex immunoassays
Contact: helena.skalnikova@lf1.cuni.cz
- Sartorius Incucyte SX1 – cell analysis, drug toxicity
Contact: peter.draber@lf1.cuni.cz
- Agilent Seahorse XFp Anylyzer – cell metabolism
Contact: peter.draber@lf1.cuni.cz
- Beckman-Coulter Cytoflex Plate Loader DW – flow cytometry
Contact: ondrej.havranek@lf1.cuni.cz
- Laser scanner Typhoon – gel electrophoresis, immunoblotting
Contact: jpetr@lf1.cuni.cz
- Sony MA900 Multi-Application Cell Sorter – flow cytometry with cell sorting
Contact: nikola.ternerova@lf1.cuni.cz
- BD FACSVerse Cell Analyzer – flow cytometry
Contact: nikola.ternerova@lf1.cuni.cz
- Agilent BioTek Cytation 5 – cell imaging multimode reader with BioTek BioSpa live cell analysis system
Contact: petr.busek2@lf1.cuni.cz
- Leica STELLARIS 5 Confocal Microscope Platforms – powerful imaging and microscopy
Contact: petr.busek2@lf1.cuni.cz
- QuantStudio 12K Flex Real-Time PCR System – real-time PCR
Contact: mhoud@lf1.cuni.cz
- HypoxyLab – incubation in hypoxia
Contact: petr.busek2@lf1.cuni.cz
Second Faculty of Medicine
- Cytometr CyTOF XT System – flow cytometry measurement
Contact: tomas.kalina@lfmotol.cuni.cz
- NextSeq 2000 System – next generation sequencing
Contact: eva.fronkova@lfmotol.cuni.cz
Faculty of Medicine in Pilsen
- Cytation C10 (Confocal Imaging Reader) – spectrometers, imaging systems, image analyzers
Contact: vladimira.moulisova@lfp.cuni.cz
Faculty of Science
- Incucyte S3, Live-Cell Analysis System – microscopy system for the automatic analysis of cell proiferation, migration and invasiveness in a medium throughput format
Contact: ondrej.tolde@natur.cuni.cz
INSTITUTE OF BIOTECHNOLOGY OF THE CZECH ACADEMY OF SCIENCES
- Modular fluorescence spectrometer FLS1000 – characterisation of molecular state in solution
Contact: hana.zambarda@ibt.cas.cz
- FTIR spectrometer Vertex 70v – characterisation of molecular state in solution
Contact: hana.zambarda@ibt.cas.cz
- timsTOF Pro mass spectrometer – MS proteomics
Contact: hana.zambarda@ibt.cas.cz
- SAXSpoint 2.0 – small angle X-ray scattering
Contact: hana.zambarda@ibt.cas.cz
- 15T-SolariX XR FT-ICR mass spectrometer – structural mass spectrometry
Contact: hana.zambarda@ibt.cas.cz
INSTITUTE OF MICROBIOLOGY OF THE CZECH ACADEMY OF SCIENCES
- Microbeta2 2450 Microplate Counter – advanced radiometric and luminescent detection abilities
Contact: makovar@biomed.cas.cz
INSTITUTE OF EXPERIMENTAL MEDICINE OF THE CZECH ACADEMY OF SCIENCES
- iScan System – high-throughput, multiplex human genotyping microarrays allow for whole-genome, targeted genome, and exome analyses, with ready-to-use expert-defined content or custom content, to suit specific research needs
Contact: pavel.rossner@iem.cas.cz
INSTITUTE OF PHOTONICS AND ELECTRONICS OF THE CZECH ACADEMY OF SCIENCES
- FEI, Versa 3D – electron microscope (SEM) and ion lithograph (FIB)
Contact: bockova@ufe.cz
- Raith, e_LiNE plus – electron lithograph (EBL) and microscope (SEM)
Contact: bockova@ufe.cz
- Pfeiffer vacuum, PLS 570L – thin film deposition apparatus for thermal evaporation in vacuum
Contact: bockova@ufe.cz
- Nanoscribe, Photonic professional GT2 – high-resolution 3D printer for ultra-precise and rapid microfabrication
Contact: bockova@ufe.cz
INSTITUTE OF ORGANIC CHEMISTRY AND BIOCHEMISTRY OF THE CZECH ACADEMY OF SCIENCES
- Magnetic cell separator MACS – isolation of specific cell populations from complex biological mixtures
Contact: tereza.ormsby@uochb.cas.cz
- OryxNano Douglas Instruments – crystallization robot that will be used to set 96well plates for crystallization screenings
Contact: tereza.vuckova@uochb.cas.cz
INSTITUTE OF MOLECULAR GENETICS OF THE CZECH ACADEMY OF SCIENCES
- Illumina NextSeq 2000 sequencer – DNA sequencing
Contact: coregenomics@img.cas.cz
- Oxford Nanopore Minion Mk1b sequencer – DNA sequencing
Contact: coregenomics@img.cas.cz
- 10x Genomics Chromium Controller microfluidics – microfluidics
Contact: coregenomics@img.cas.cz
Agilent Bioanalyzer 2100 capillary electrophoresis – capillary electrophoresis
Contact: coregenomics@img.cas.cz
- Agilent Fragment Analyzer 5200 capillary electrophoresis – capillary electrophoresis
Contact: coregenomics@img.cas.cz
- Formulatrix Mantis automated liquid dispenser – liquid manipulation
Contact: coregenomics@img.cas.cz
- Bio-Rad TC20 automated cell counter – cell counter
Contact: coregenomics@img.cas.cz
- Amnis ImageStream X Mk II – imaging flow cytometer
Contact: ondrej.horvath@img.cas.cz
- BC-30 Vet Auto Hematology Analyzer – cell counter
Contact: ondrej.horvath@img.cas.cz
- BD Influx – cell sorter
Contact: ondrej.horvath@img.cas.cz
- FACSAria IIu – cell sorter
Contact: ondrej.horvath@img.cas.cz
- Automated cell separator AutoMACS Pro – cell separator
Contact: ondrej.horvath@img.cas.cz
- Leica TCS SP5 AOBS Tandem – confocal laser scanning microscopy
Contact: ondrej.horvath@img.cas.cz
- Leica TCS SP8 – confocal laser scanning microscopy
Contact: ondrej.horvath@img.cas.cz
- Leica STELLARIS 8 FALCON with the LIGHTNING adaptive deconvolution – confocal scanning fluorescence imaging with Lightning superresolution module
Contact: ondrej.horvath@img.cas.cz
- Andor Dragonfly 503 – spinning disk confocal microscope, equipped with microinjection
Contact: ondrej.horvath@img.cas.cz
- Olympus ScanR high content microscope – high-content imaging
Contact: ondrej.horvath@img.cas.cz
- Zeiss AxioZoom.V16 macroscope with Apotome – fluorescence imaging on widefield optical sectioning mode
Contact: ondrej.horvath@img.cas.cz
- Zeiss Lightsheet Z.1 microscope – fluorescence imaging with lightsheet optical sectioning
Contact: ondrej.horvath@img.cas.cz
- Leica TCS SP8 STED 3X – confocal scanning fluorescence imaging with STED superresolution module
Contact: ondrej.horvath@img.cas.cz
- DeltaVision OMX V4 – widefield fluorescence imaging with SIM or DSSI superresolution module
Contact: ondrej.horvath@img.cas.cz
- Zeiss Elyra 7 – single molecule localization microscopy (3D PALM / dSTORM)
Contact: ondrej.horvath@img.cas.cz
- TESCAN Q-PHASE – holographic microscope for quantitative phase imaging
Contact: ondrej.horvath@img.cas.cz
- Jeol JEM-F200 “F2” Transmission Electron Microscope – highly advanced state-of-the-art 200kV S/TEM electron microscope
Contact: vlada.filimonenko@img.cas.cz
- Jeol JEM-1400 FLASH Transmission Electron Microscope – electron microscope with a tungsten or LaB6 filament electron beam
Contact: vlada.filimonenko@img.cas.cz
- Orbitrap Exploris 480 mass spectrometer – mass spectrometry
Contact: miluse.hradilova@img.cas.cz
- nano UHPLC Ultimate 3000 liquid chromatograph – liquid chromatography/mass spectrometery
Contact: miluse.hradilova@img.cas.cz
MASARYK UNIVERSITY
Faculty of Science
- Confocal microscope Leica TCS SP8 with live imaging equipment – confocal microscopy, live imaging
Contact: bryja@sci.muni.cz; slabakova@sci.muni.cz
- Flow cytometer Northern Lights NL-3000 V/B/R-41 – spectral flow cytometry
Contact: bryja@sci.muni.cz; slabakova@sci.muni.cz
- Fourier Transform Infrared Spectrometer INVENIO X Bruker – protein engineering
Contact: kovard@mail.muni.cz; zbynek@chemi.muni.cz
- Agilent HPLC 1260 (DAD, FLD) – protein engineering, analytical biochemistry
Contact: kovard@mail.muni.cz; zbynek@chemi.muni.cz
- Circular Dichroism spectrometer Chirascan – protein engineering, analytical biochemistry
Contact: kovard@mail.muni.cz; zbynek@chemi.muni.cz
- Systems Stopped/Quench-Flow BioLogic (DAD, FLD, CD) – protein engineering, analytical biochemistry
Contact: kovard@mail.muni.cz; zbynek@chemi.muni.cz
- GC-MS Agilent 7890A + 5975C – protein engineering, analytical biochemistry
Contact: kovard@mail.muni.cz; zbynek@chemi.muni.cz
- 3D Scanning Laser Microscope LEXT OLS4100 Olympus – scanning surfaces
Contact: kovard@mail.muni.cz; zbynek@chemi.muni.cz
- UNCLE (stability screening platform FLD, SLS, DLS) – protein engineering, analytical biochemistry
Contact: kovard@mail.muni.cz; zbynek@chemi.muni.cz
- High-sensitive BL700UHS Bioluminescent Microscope PSI – protein engineering, analytical biochemistry, microfluidics
Contact: kovard@mail.muni.cz; zbynek@chemi.muni.cz
- ODIN High-speed camera – protein engineering, analytical biochemistry, microfluidics
Contact: kovard@mail.muni.cz; zbynek@chemi.muni.cz
- Laser densitometer Pharos FX Pro – gel electrophoresis with labeling by fluorophores
Contact: bouchal@chemi.muni.cz
CEITEC
- Illumina sequencers (MiSeq, NextSeq 500, NovaSeq 6000) – massively parallel sequencing
Contact: cfg@ceitec.muni.cz
- Flow cytometer (BD Verse), Cell sorter (BD Aria Fusion) – immunophenotyping, cell sorting
Contact: tomas.loja@ceitec.muni.cz
- Single-cell sequencing (10X Chromium) – single-cell RNAseq library preparation
Contact: cfg@ceitec.muni.cz
MASARYK MEMORIAL CANCER INSTITUTE
- QX200 Droplet Digital PCR System – genomics (absolute quantification of DNA)
Contact: hrstka@mou.cz
- Orbitrap Fusion mass spectrometer – proteomics (LC-MS/MS)
Contact: lenka.hernychova@mou.cz
- timsTOF SCP mass spectrometer – proteomics (LC-MS/MS)
Contact: lenka.hernychova@mou.cz
PALACKÝ UNIVERSITY
Institute of Molecular and Translational Medicine
- AB SCIEX QTRAP 5500 Tandem mass spectrometer – mass spectrometers, separation systems
Contact: marian.hajduch@upol.cz
- AB SCIEX mass spectrometer TripleTOF 5600+ – mass spectrometer
Contact: marian.hajduch@upol.cz
- BD FACSAria II Sorpe – flow cytometer/sorter
Contact: marian.hajduch@upol.cz
- BD FACSCalibur – flow cytometer
Contact: marian.hajduch@upol.cz
- Biological samples irradiator (Cesium-137)
Contact: marian.hajduch@upol.cz
- Bruker ultrafleXtreme MALDI-TOF/TOF – mass spectrometer
Contact: marian.hajduch@upol.cz
- Carl Zeiss LSM 780 with UV laser and ELYRA.PS1 module – confocal microscope
Contact: marian.hajduch@upol.cz
- GE Typhoon FLA9000 – laser scanner for biomolecular imaging
Contact: marian.hajduch@upol.cz
- HighRes Biosciences Robotic platform for ultra-high-throughput testing of biological activity of small molecules
Contact: marian.hajduch@upol.cz
- HighRes Biosolutions Inc. robotic system for storage of chemical libraries and preparation of test panels of these chemicals
Contact: marian.hajduch@upol.cz
- Leco Pegasus 4D Orthogonal gas chromatograph (GCxGC)
Contact: marian.hajduch@upol.cz
- MiSeq – sequencing platform
Contact: marian.hajduch@upol.cz
- NanoScan PET/MRI 3T Mediso – documentation, imaging systems, image analyzers
Contact: marian.hajduch@upol.cz
- NovaSeq 6000 – sequencing platform
Contact: marian.hajduch@upol.cz
- Perkin Elmer Sciclone G3 NGSx iQ – automatic pipeting station
Contact: marian.hajduch@upol.cz
- PerkinElmer 2480 WIZARD 2 – automatic gamma counter
Contact: marian.hajduch@upol.cz
- PerkinElmer Columbus Conductor 24TB – system for image data analysis and management
Contact: marian.hajduch@upol.cz
- PerkinElmer Cyclone Plus Storage Phosphor System multimodal scanner
Contact: marian.hajduch@upol.cz
- PerkinElmer EnVision HTS Multilabel Plate Reader – documentation, imaging systems, image analyzers
Contact: marian.hajduch@upol.cz
- PerkinElmer EnVision HTS Multilabel Plate Reader with AlphaScreen Microplate reader – documentation, imaging systems, image analyzers
Contact: marian.hajduch@upol.cz
- PerkinElmer Enspire Microwell plate reader – documentation, imaging systems, image analyzers
Contact: marian.hajduch@upol.cz
- PerkinElmer MicroBeta – radioactivity and luminescence counter
Contact: marian.hajduch@upol.cz
- PerkinElmer Operetta imaging system
Contact: marian.hajduch@upol.cz
- PerkinElmer microplates reader ViewLux uHTS Microplate Imager
Contact: marian.hajduch@upol.cz
- Preparative HPLC – separation systems
Contact: marian.hajduch@upol.cz
- WITec alpha300 R + Confocal Raman imaging microscope system
Contact: marian.hajduch@upol.cz
- Yokogawa CV7000 Voyager Cell High throughput cellular imaging and discovery system
Contact: marian.hajduch@upol.cz
- Zeiss LSM980 – microscopic systems
Contact: marian.hajduch@upol.cz