Martin Sztacho

Laboratory of cancer cell architecture

Head: Martin Sztacho
Prague node

martin.sztacho@lf1.cuni.cz
website of the Institute

HOME INSTITUTION

First Faculty of Medicine CU

RESEARCH PROGRAMME(S)

RP 1 - Molecular basis of cancer and molecular targets

Let's beat cancer by understanding cellular architecture!

COLLABORATION WITH INFRASTRUCTURES

Mission: Investigating the molecular mechanisms of pathophysiological changes in cellular architecture during tumorigenesis.
Vision: To identify the key factors that regulate the formation of pathological structures and prevent their formation in cancer cells.

The spatiotemporal organization of biological matter is an important determinant of the efficacy and rate of cellular processes. In the newly established (February 2024) Laboratory of Cancer Cell Architecture, we investigate the physicochemical aspects of the subcellular localization of biomolecular material during the malignant manifestations of cancer transformation. Understanding how the cellular subcompartmentalized environment is organized and maintained in health and disease is essential for early diagnosis and effective treatment of cancer. Therefore, the aim of our research is to gain insight into the molecular mechanisms of pathophysiological changes in the cellular architecture during oncogenesis. In particular, we are interested in understanding the principles of biomolecular condensation through the process of phase separation affected by phosphoinositides and RNA molecules.

SELECTED PUBLICATIONS

Hoboth P, Sztacho M, Hozák P. Nuclear patterns of phosphatidylinositol 4,5- and 3,4-bisphosphate revealed by super-resolution microscopy differ between the consecutive stages of RNA polymerase II transcription. FEBS J. 2024 May 11. DOI: 10.1111/febs.17136
Koudelková L, Pelantová M, Brůhová Z, Sztacho M, Pavlík V, Pánek D, Gemperle J, Talacko P, Brábek J, Rösel D. Phosphorylation of tyrosine 90 in SH3 domain is a new regulatory switch controlling Src kinase. Elife. 2023 Jul 10;12:e82428. DOI: 10.7554/eLife.82428
Hoboth P, Sztacho M, Šebesta O, Schätz M, Castano E, Hozák P. Nanoscale mapping of nuclear phosphatidylinositol phosphate landscape by dual-color dSTORM. Biochim Biophys Acta Mol Cell Biol Lipids. 2021. DOI: 10.1016/j.bbalip.2021.158890
Sztacho M, Šalovská B, Červenka J, Balaban C, Hoboth P, Hozák P, Limited Proteolysis-Coupled Mass Spectrometry Identifies Phosphatidylinositol 4,5-Bisphosphate Effectors in Human Nuclear Proteome. Cells. 2021. DOI: 10.3390/cells10010068
Dudley LJ, Cabodevilla AG, Makar AN, Sztacho M, Michelberger T, Marsh JA, Houston DR, Martens S, Jiang X, Gammoh N. Intrinsic lipid binding activity of ATG16L1 supports efficient membrane anchoring and autophagy. EMBO J. 2019. DOI: 10.15252/embj.2018100554