Laboratory of Cell and Developmental Biology (LCDB)
Head: Vladimír Kořínek
Prague node
HOME INSTITUTION
Institute of Molecular Genetics of the Czech Academy of Sciences
RESEARCH PROGRAMME(S)
RP 1 - Molecular basis of cancer and molecular targets
RP 3 - Biomarkers of cancers and cancer diagnostics
We uncover the molecular and genetic basis of intestinal and blood cancer cells.
- Mission: Analysis of the properties of cancer driver genes in different types of biological models
- Vision: Our knowledge will expand the possibilities of targeted cancer therapy
Most types of cancer arise and develop due to genetic changes whose interactions lead to malignant stages. Tumors consist of subclones of cancer cells, and the mutations in the genomic DNA of these subclones can be very different. However, the genetic basis, i.e. the group of key mutations, was originally the same in all tumor cells. The main goal of the LCDB is to find out how specific genetic and associated molecular changes influence the properties of healthy and cancer cells. The LCDB focuses on analyzing the so-called cancer (driver) genes and investigates their functions. The long-term goal is to use the newly gained knowledge for the diagnosis or targeted therapy of selected malignant diseases.
It is important to emphasize that the LCDB has unique expertise in human disease modeling and in the use of sophisticated in vitro models such as organoids and induced pluripotent stem cells. Although the LCDB’s main priorities remain basic research, it is keen to capture and promote the translational potential of the results obtained. The LCDB sees its participation in the consortium as an excellent opportunity to significantly strengthen biomedical and preclinical research and to network organically with other oncology-oriented institutions in the Czech Republic.
SELECTED PUBLICATIONS
- Berková L, Fazilaty H, Yang Q, Kubovčiak J, Stastna M, Hrckulak D, Vojtechova M, Dalessi T, Brügger MD, Hausmann G, Liberali P, Korinek V, Basler K, Valenta T. Terminal differentiation of villus tip enterocytes is governed by distinct Tgfβ superfamily members. EMBO Rep. 2023 Sep 6;24(9):e56454. DOI: 10.15252/embr.202256454
- Švec J, Šťastná M, Janečková L, Hrčkulák D, Vojtěchová M, Onhajzer J, Kříž V, Galušková K, Šloncová E, Kubovčiak J, Pfeiferová L, Hrudka J, Matěj R, Waldauf P, Havlůj L, Kolář M, Kořínek V. TROP2 Represents a Negative Prognostic Factor in Colorectal Adenocarcinoma and Its Expression Is Associated with Features of Epithelial-Mesenchymal Transition and Invasiveness. Cancers (Basel). 2022 Aug 26;14(17):4137. DOI: 10.3390/cancers14174137
- Danek P, Kardosova M, Janeckova L, Karkoulia E, Vanickova K, Fabisik M, Lozano-Asencio C, Benoukraf T, Tirado-Magallanes R, Zhou Q, Burocziova M, Rahmatova S, Pytlik R, Brdicka T, Tenen DG, Korinek V, Alberich-Jorda M. β-Catenin-TCF/LEF signaling promotes steady-state and emergency granulopoiesis via G-CSF receptor upregulation. Blood. 2020 Nov 26;136(22):2574-2587. DOI: 10.1182/blood.2019004664
- Tumova L, Pombinho AR, Vojtechova M, Stancikova J, Gradl D, Krausova M, Sloncova E, Horazna M, Kriz V, Machonova O, Jindrich J, Zdrahal Z, Bartunek P, Korinek V. Monensin inhibits canonical Wnt signaling in human colorectal cancer cells and suppresses tumor growth in multiple intestinal neoplasia mice. Mol Cancer Ther. 2014 Apr;13(4):812-22. DOI: 10.1158/1535-7163.MCT-13-0625
- Fafilek B, Krausova M, Vojtechova M, Pospichalova V, Tumova L, Sloncova E, Huranova M, Stancikova J, Hlavata A, Svec J, Sedlacek R, Luksan O, Oliverius M, Voska L, Jirsa M, Paces J, Kolar M, Krivjanska M, Klimesova K, Tlaskalova-Hogenova H, Korinek V. Troy, a tumor necrosis factor receptor family member, interacts with lgr5 to inhibit wnt signaling in intestinal stem cells. Gastroenterology. 2013 Feb;144(2):381-391. DOI: 10.1053/j.gastro.2012.10.048
COLLABORATION WITHIN THE NICR
Meritxell Alberich Jordà
(Prague node, Institute of Molecular Genetics of the Czech Academy of Sciences)
Libor Macůrek
(Prague node, Institute of Molecular Genetics of the Czech Academy of Sciences)
Martin Gregor
(Prague node, Institute of Molecular Genetics of the Czech Academy of Sciences)
Michal Kolář
(Prague node, Institute of Molecular Genetics of the Czech Academy of Sciences)
Vladimír Divoký
(Olomouc node, Faculty of Medicine PU in Olomouc)
SPECIALIZED EXPERTISE AND TECHNOLOGY
Production and use of genetically modified mouse models
Use of organoids to model tissue growth and development “in vitro”
Advanced “omics” analysis of tissues at the single-cell level (e.g. single-cell expression profiling)
COLLABORATION WITH LARGE RESEARCH INFRASTRUCTURES AND RESEARCH CENTRES
National infrastructure for biological and biomedical imaging Czech-Bioimaging
Czech Centre for Phenogenomics
Biotechnology and Biomedicine Centre of the Czech Academy of Sciences and the Charles University BIOCEV