Laboratory of Biomolecular Recognition
Head: Bohdan Schneider
Prague node
HOME INSTITUTION
Institute of Biotechnology of the Czech Academy of Sciences
RESEARCH PROGRAMME(S)
RP 2 - Research and development of anticancer pharmaceuticals and therapeutic methods
Designing new proteins to find new drugs.
Our main research goal within the NICR project is the design, development and testing of new protein constructs targeting oncologically relevant targets such as cell receptors with a focus on the role of T, NK and Treg immune populations in the treatment of oncological diseases.
When designing new proteins, we use standard tools of sequence and structural bioinformatics and computational methods of molecular modeling, including molecular dynamics and related empirical potential methods. Experimental protein development relies on directed evolution methods, especially ribosome display and yeast display. In these methods, we use original protein scaffolds developed in the laboratory, and therefore their eventual commercial application cannot conflict with patent protection from foreign entities.
In the project, we plan to use our experience with the development and testing of cytokines and proteins of innate immunity. We target both these signaling molecules and their cellular receptors. We focus on the signaling systems of pro-inflammatory interferon lambda, interleukin 9, or anti-inflammatory interleukin 10.
SELECTED PUBLICATIONS
- Huličiak M, Biedermannová L, Berdár D, Herynek Š, Kolářová L, Tomala J, Mikulecký P, Schneider B: Combined in vitro and cell-based selection display method producing specific binders against IL-9 receptor in high yields. FEBS J. 290: 2993-3005 (2023). DOI: 10.1111/febs.16726
- Pham PN, Zahradník J, Kolářová L, Schneider B, Fuertes G: Regulation of IL-24/IL-20R2 complex formation using photocaged tyrosines and UV light. Frontiers in Molecular Biosciences. 10: 1214235 (2023). DOI: 10.3389/fmolb.2023.1214235
- Kolářová L, Zahradník J, Huličiak M, Mikulecký P, Peleg Y, Shemesh M, Schreiber G, Schneider B. De novo developed protein binders mimicking Interferon lambda signaling. FEBS J. 2022 May;289(9):2672-2684. DOI: 10.1111/febs.16300
- Pham PN, Huličiak M, Biedermannová L, Černý J, Charnavets T, Fuertes G, Herynek Š, Kolářová L, Kolenko P, Pavlíček J, Zahradník J, Mikulecky P, Schneider B. Protein Binder (ProBi) as a New Class of Structurally Robust Non-Antibody Protein Scaffold for Directed Evolution. Viruses. 2021 Jan 27;13(2):190. DOI: 10.3390/v13020190
- Zahradník J, Kolářová L, Peleg Y, Kolenko P, Svidenská S, Charnavets T, Unger T, Sussman JL, Schneider B. Flexible regions govern promiscuous binding of IL-24 to receptors IL-20R1 and IL-22R1. FEBS J. 2019 Oct;286(19):3858-3873. DOI: 10.1111/febs.14945
COLLABORATION WITHIN THE NICR
Cyril Bařinka
(Prague node, Institute of Biotechnology of the Czech Academy of Sciences)
SPECIALIZED EXPERTISE AND TECHNOLOGY
Sudy specific protein-protein and protein-DNA interactions
Experimental and computational methods of protein engineering
Methods of structural biology, bioinformatics, and molecular modeling
COLLABORATION WITH LARGE RESEARCH INFRASTRUCTURES AND RESEARCH CENTRES
Biotechnology and Biomedicine Centre of the Czech Academy of Sciences and the Charles University BIOCEV
Czech Infrastructure for Integrative Structural Biology CIISB
Czech National Infrastructure for Biologicl Data ELIXIR CZ
Research Infrastructure in Structural Biology Instruct-ERIC