Laboratory of cancer cell biology (LCCB)
Head: Aleksi Šedo
Prague node
HOME INSTITUTION
First Faculty of Medicine CU
RESEARCH PROGRAMME(S)
RP 1 - Molecular basis of cancer and molecular targets
Watch out, dude! A tumor is not just cancer cells!
- Mission: To search for pathogenetically relevant processes that drive brain tumour progression
- Vision: To identify and target therapeutically or diagnostically relevant cellular and molecular targets in primary and secondary brain tumors
LCCB focuses on the role of plasma membrane-bound proteases in various physiological and pathophysiological processes, particularly in the pathogenesis of cancer. In addition to focused research on intercellular interactions in the tumor microenvironment that drive tumor growth, neoangiogenesis, and dysregulation of the anti-tumor local and systemic immune response, our translational projects focus on the development of innovative diagnostic (imaging) and therapeutic concepts exploiting different molecular targets, particularly in glioblastomas and brain metastases.
In collaboration with other groups in the consortium, we are developing and testing novel compounds to influence the behaviour of cell populations present in the tumour microenvironment and to use identified molecular targets for diagnostic or therapeutic applications.
SELECTED PUBLICATIONS
- Vymola P, Garcia-Borja E, Cervenka J, Balaziova E, Vymolova B, Veprkova J, Vodicka P, Skalnikova H, Tomas R, Netuka D, Busek P, Sedo A. : Fibrillar extracellular matrix produced by pericyte-like cells facilitates glioma cell dissemination. Brain Pathol. 2024 May 5:e13265. DOI: 10.1111/bpa.13265
- Konečná D, Výmola P, Ternerová N, Výmolová B, Garcia-Borja E, Mateu R, Šroubek F, Pankrác J, Widen JC, Bogyo M, Netuka D, Bušek P, Šedo A.: Molecularly targeted protease-activated probes for visualization of glioblastoma: a comparison with 5-ALA. J Neurosurg. 2024 Mar 29:1-12. DOI: 10.3171/2024.1.JNS231137
- Zubaľ M, Výmolová B, Matrasová I, Výmola P, Vepřková J, Syrůček M, Tomáš R, Vaníčková Z, Křepela E, Konečná D, Bušek P, Šedo A.: Fibroblast activation protein as a potential theranostic target in brain metastases of diverse solid tumours. Pathology. 2023 Jun 16:S0031-3025(23)00137-X. DOI: 10.1016/j.pathol.2023.05.003
- Dvořáková P, Bušek P, Knedlík T, Schimer J, Etrych T, Kostka L, Stollinová Šromová L, Šubr V, Šácha P, Šedo A, Konvalinka J: Inhibitor-Decorated Polymer Conjugates Targeting Fibroblast Activation Protein. J Med Chem. 2017 Oct 26;60(20):8385-8393. DOI: 10.1021/acs.jmedchem.7b00767
- Sedo A. and Mentlein R (eds.). Glioma Cell Biology. Book. Springer, 2014. ISBN 978-3-7091-1430-8
COLLABORATION WITHIN THE NICR
Jan Brábek
(Prague node, Faculty of Science CU)
Karel Smetana
(Prague node, First Faculty of Medicine CU)
Helena Kupcová Skalníková
(Prague node, First Faculty of Medicine CU)
Marek Kovář
(Prague node, Institute of Microbiology of the Czech Academy of Sciences)
Martin Sztacho
(Prague node, First Faculty of Medicine CU)
Michal Kolář
(Prague node, Institute of Molecular Genetics of the Czech Academy of Sciences)
Milan Jakubek
(Prague node, First Faculty of Medicine CU)
Ondřej Slabý
(Brno node, Faculty of Medicine MU/CEITEC MU)
Tomáš Etrych
(Prague node, Institute of Macromolecular Chemistry of the Czech Academy of Sciences)
SPECIALIZED EXPERTISE AND TECHNOLOGY
Advanced biological models, 2D, 3D, animal, stereotactic implantation
Cell biology methods portfolio
Live cell confocal microscopy, flow cytometry, sorting
COLLABORATION WITH LARGE RESEARCH INFRASTRUCTURES AND RESEARCH CENTRES
National infrastructure for translational medicine EATRIS-CZ
National infrastructure for biological and biomedical imaging Czech-Bioimaging
Biotechnology and Biomedicine Centre of the Czech Academy of Sciences and the Charles University BIOCEV
Central European Institute of Technology at Masaryk University CEITEC MU