Laboratory of Haematooncology and Stem Cells
Head: Tomáš Stopka
Prague node
HOME INSTITUTION
First Faculty of Medicine CU
RESEARCH PROGRAMME(S)
RP 5 - Translational oncology: Verification clinical studies of the proof-of-concept type
Stem cells are the mastermind of the tumor!
The progression of leukemias is related to tumor cell resistance, which is an ideal target for new therapies, but specific molecules that drive resistance must not only be discovered and validated in mouse PDX models, but have to move via clinical studies towards patient‘s bed side!
We are physicians, biologists and mathematicians that are interested in the clonal evolution of tumours, in particular the ability of cancer stem cells to escape targeted therapies. We study how resistance leads to reprogramming of gene expression, changes in metabolism, but also how it affects the tuning of other processes including redox homeostasis.
We use state-of-the-art technologies such as targeted proteomics, genomics, and transgenic research in the mouse. We can also track natural or pathological protein modifications and use these technologies to identify molecular processes associated with the development and progression of malignancies and the emergence of resistance.
We collaborate within the NIRC but also with scientists from around the world, educate students and participate in clinical applied projects and patient organizations.
SELECTED PUBLICATIONS
- Turkova T, Kokavec J, Zikmund T, Dibus N, Pimkova K, Nemec D, Holeckova M, Ruskova L, Sedlacek R, Cermak L, Stopka T. Differential requirements for Smarca5 expression during hematopoietic stem cell commitment. Commun Biol. 2024 Feb 29;7(1):244. DOI: 10.1038/s42003-024-05917-z
- Stopka T, Minařík L, Dusilková N, Pešta M, Kulvait V, Špaček M, Zemanová Z, Kalousová M, Jonášová A. G-CSF plus azacitidine versus azacitidine alone for patients with high-risk myelodysplastic syndrome: academic, open label, randomized trial. Blood Cancer J. 2022 Jul 7;12(7):105. DOI: 10.1038/s41408-022-00698-2
- Kokavec J, Zikmund T, Savvulidi F, Kulvait V, Edelmann W, Skoultchi AI, Stopka T. The ISWI ATPase Smarca5 (Snf2h) Is Required for Proliferation and Differentiation of Hematopoietic Stem and Progenitor Cells. Stem Cells. 2017 Jun;35(6):1614-1623. DOI: 10.1002/stem.2604
- Janostiak R, Rauniyar N, Lam TT, Ou J, Zhu LJ, Green MR, Wajapeyee N. MELK Promotes Melanoma Growth by Stimulating the NF-κB Pathway. Cell Rep. 2017 Dec 5;21(10):2829-2841. DOI: 10.1016/j.celrep.2017.11.033
- Petrak J, Ivanek R, Toman O, Cmejla R, Cmejlova J, Vyoral D, Zivny J, Vulpe CD. Déjà vu in proteomics. A hit parade of repeatedly identified differentially expressed proteins. Proteomics. 2008 May;8(9):1744-9. DOI: 10.1002/pmic.200700919
COLLABORATION WITHIN THE NICR
Marek Mráz
(Brno node, CEITEC MU)
Milan Jakubek
(Prague node, First Faculty of Medicine CU)
Pavel Klener
(Prague node, First Faculty of Medicine CU)
Vladimír Divoký
(Olomouc node, Faculty of Medicine PU in Olomouc)
Zdeněk Hodný
(Prague node, Institute of Molecular Genetics of the Czech Academy of Sciences)
SPECIALIZED EXPERTISE AND TECHNOLOGY
Live cell confocal microscopy, flow cytometry, sorting
Proteomic analysis of cells, tissues and body fluids using isotope labeling methods iTRAQ, TMT or SILAC
Antibody-based detection and quantitation (western blotting and ELISA)
Next-generation sequencing
Tissue cryopreservation and cell separation