Laboratory of cancer cell architecture
Head: Martin Sztacho
Prague node
HOME INSTITUTION
First Faculty of Medicine CU
RESEARCH PROGRAMME(S)
RP 1 - Molecular basis of cancer and molecular targets
Let's beat cancer by understanding cellular architecture!
- Mission: Investigating the molecular mechanisms of pathophysiological changes in cellular architecture during tumorigenesis.
- Vision: To identify the key factors that regulate the formation of pathological structures and prevent their formation in cancer cells.
The spatiotemporal organization of biological matter is an important determinant of the efficacy and rate of cellular processes. In the newly established (February 2024) Laboratory of Cancer Cell Architecture, we investigate the physicochemical aspects of the subcellular localization of biomolecular material during the malignant manifestations of cancer transformation. Understanding how the cellular subcompartmentalized environment is organized and maintained in health and disease is essential for early diagnosis and effective treatment of cancer. Therefore, the aim of our research is to gain insight into the molecular mechanisms of pathophysiological changes in the cellular architecture during oncogenesis. In particular, we are interested in understanding the principles of biomolecular condensation through the process of phase separation affected by phosphoinositides and RNA molecules.
SELECTED PUBLICATIONS
- Hoboth P, Sztacho M, Hozák P. Nuclear patterns of phosphatidylinositol 4,5- and 3,4-bisphosphate revealed by super-resolution microscopy differ between the consecutive stages of RNA polymerase II transcription. FEBS J. 2024 May 11. DOI: 10.1111/febs.17136
- Koudelková L, Pelantová M, Brůhová Z, Sztacho M, Pavlík V, Pánek D, Gemperle J, Talacko P, Brábek J, Rösel D. Phosphorylation of tyrosine 90 in SH3 domain is a new regulatory switch controlling Src kinase. Elife. 2023 Jul 10;12:e82428. DOI: 10.7554/eLife.82428
- Hoboth P, Sztacho M, Šebesta O, Schätz M, Castano E, Hozák P. Nanoscale mapping of nuclear phosphatidylinositol phosphate landscape by dual-color dSTORM. Biochim Biophys Acta Mol Cell Biol Lipids. 2021. DOI: 10.1016/j.bbalip.2021.158890
- Sztacho M, Šalovská B, Červenka J, Balaban C, Hoboth P, Hozák P, Limited Proteolysis-Coupled Mass Spectrometry Identifies Phosphatidylinositol 4,5-Bisphosphate Effectors in Human Nuclear Proteome. Cells. 2021. DOI: 10.3390/cells10010068
- Dudley LJ, Cabodevilla AG, Makar AN, Sztacho M, Michelberger T, Marsh JA, Houston DR, Martens S, Jiang X, Gammoh N. Intrinsic lipid binding activity of ATG16L1 supports efficient membrane anchoring and autophagy. EMBO J. 2019. DOI: 10.15252/embj.2018100554
COLLABORATION WITHIN THE NICR
Aleksi Šedo
(Prague node, First Faculty of Medicine CU)
Jan Brábek
(Prague node, Faculty of Science CU)
Helena Kupcová Skalníková
(Prague node, First Faculty of Medicine CU)
Michal Kolář
(Prague node, Institute of Molecular Genetics of the Czech Academy of Sciences)
Zdeněk Hodný
(Prague node, Institute of Molecular Genetics of the Czech Academy of Sciences)
SPECIALIZED EXPERTISE AND TECHNOLOGY
Advanced microscopy
Tissue culture
Cell biology
Protein purification
COLLABORATION WITH LARGE RESEARCH INFRASTRUCTURES AND RESEARCH CENTRES
National infrastructure for biological and biomedical imaging Czech-Bioimaging
Biotechnology and Biomedicine Centre of the Czech Academy of Sciences and the Charles University BIOCEV
Yucatán Centre for Scientific Research A.C.
Translational Research for Infectious Diseases and Oncology Research Center TRIO