Non-Coding Genome
Head: Lukáš Trantírek
Brno node
HOME INSTITUTION
CEITEC MU
RESEARCH PROGRAMME(S)
RP 1 - Molecular basis of cancer and molecular targets
RP 3 - Biomarkers of cancers and cancer diagnostics
With programmed cell death, DNA fragments are released. The released DNA fragments have preserved the genetic and epigenetic code of the cell of origin. The fragmentation of the released extracellular DNA (eDNA) in the blood plasma reflects the tissue origin of eDNA and the mechanism of its release.
eDNA is an important diagnostic, prognostic or predictive biomarker for various types of cancer: Blood plasma from patients with metastases generally shows higher levels of eDNA than in healthy individuals. eDNA levels decrease in response to successful therapy, while remaining constant or increasing in situations that are associated with tumor recurrence or worsening patient condition. Until now, however, eDNA-based approaches have primarily focused on the analysis of double-stranded DNA (dsDNA).
SELECTED PUBLICATIONS
- Krafčík D, Ištvánková E, Džatko Š, Víšková P, Foldynová-Trantírková S, Trantírek L. Towards Profiling of the G-Quadruplex Targeting Drugs in the Living Human Cells Using NMR Spectroscopy. Int J Mol Sci. 2021 Jun 3;22(11):6042. DOI: 10.3390/ijms22116042
- Gregor T, Bosakova MK, Nita A, Abraham SP, Fafilek B, Cernohorsky NH, Rynes J, Foldynova-Trantirkova S, Zackova D, Mayer J, Trantirek L, Krejci P. Elucidation of protein interactions necessary for the maintenance of the BCR-ABL signaling complex. Cell Mol Life Sci. 2020 Oct;77(19):3885-3903. DOI: 10.1007/s00018-019-03397-7
- Krafcikova M, Dzatko S, Caron C, Granzhan A, Fiala R, Loja T, Teulade-Fichou MP, Fessl T, Hänsel-Hertsch R, Mergny JL, Foldynova-Trantirkova S, Trantirek L. Monitoring DNA-Ligand Interactions in Living Human Cells Using NMR Spectroscopy. J Am Chem Soc. 2019 Aug 28;141(34):13281-13285. DOI: 10.1021/jacs.9b03031
- Dzatko S, Krafcikova M, Hänsel-Hertsch R, Fessl T, Fiala R, Loja T, Krafcik D, Mergny JL, Foldynova-Trantirkova S, Trantirek L. Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells. Angew Chem Int Ed Engl. 2018 Feb 19;57(8):2165-2169. DOI: 10.1002/anie.201712284
- Renčiuk D, Ryneš J, Kejnovská I, Foldynová-Trantírková S, Andäng M, Trantírek L, Vorlíčková M. G-quadruplex formation in the Oct4 promoter positively regulates Oct4 expression. Biochim Biophys Acta Gene Regul Mech. 2017 Feb;1860(2):175-183. DOI: 10.1016/j.bbagrm.2016.11.002